Figure 2
From: Oncostatin M promotes STAT3 activation, VEGF production, and invasion in osteosarcoma cell lines
Activation of STAT3, JAK2, and Src in OSA cell lines upon following stimulation with OSM. Canine (OSA8) or human (SJSA) OSA cell lines were serum starved for two hours then stimulated with rhOSM (50 ng/mL) for 0 (no OSM stimulation), 5, 10, or 30 minutes. Protein lysates were generated and separated by SDS-PAGE and Western blotting for pSTAT3 (Y705), total STAT3, pJAK2 (Y1007/1008), total JAK2, pSrc (Y418), and total Src was performed.