Irinophore C™, liposomal vincristine and Caelyx
® treatments are associated with vascular normalization of the tumor vasculature in orthotopic GBM tumors. a) Vessel diameters (■, left axis) and the fraction of collagen IV-free CD31 pixels (■, right axis) in orthotopic GBM tumors were reduced by Irinophore C™ and Caelyx®. However, no changes were seen in the fraction of NG2-free CD31 positive endothelial cells (■, right axis) or Collagen IV-free CD31 positive endothelial cells (□, right axis). b) Representative images from untreated and Irinophore C™ treated tumors; similar images for normal brain tissue are shown for comparison. (c) Blood vessel diameters (CD31; green) are reduced by Irinophore C™ treatment. The basement membrane (collagen IV; yellow) is partially restored by treatment with Irinophore C™. The entire image represents non-necrotic and viable tissue. Statistical significances are indicated (*p-value ≤ 0.05; **p-value ≤ 0.01). Non-significant trends are indicated (&p-value = 0.064).