Orthotopic GBM tumors treated with Irinophore C™, liposomal vincristine and Caelyx
® are significantly smaller than untreated controls. a) Representative H&E images of brain sections from mice in each treatment group show that the area of tumor tissue (dark blue) from treated animals are smaller than untreated controls. b) Tumor areas were quantified in number of pixels, and used as a measure of treatment induced reduction of the tumor mass (■, left axis). No significant changes in proliferative activity (■, right axis) were observed. c) Hoechst 33342 staining was reduced significantly following treatments with the three liposomal treatments (■, left axis). The total number of endothelial cells per unit area of viable tissue (■, right axis) was unchanged across all groups, but the fraction of endothelial cells that were co-stained with Hoechst 33342 was significantly reduced (□, right axis). d) The density of endothelial cells (positive CD31 pixels divided by periphery or center tumor area pixels) in the center of tumors treated with Irinophore C™ was significantly higher compared to control tumors (□). No changes in endothelial cell density were seen in the total tumor area (■) or the periphery of tumors (■). Statistical significances are indicated (*p-value ≤ 0.05; **p-value ≤ 0.01; ***p-value ≤ 0.001). Non-significant trends are indicated (&p-value = 0.067).