Table 1 Primers for RT-PCR analysis of differentially expressed genes induced by latexin expression in MGC803 cells.
Gene symbol | Primers, 5'-3' | Annealing (°C) | Cycles (N) | Product (bp) |
|---|---|---|---|---|
S100P | Â | 59 | 24 | 161 |
F | CGTGGATAAATTGCTCAAGGAC | Â | Â | Â |
R | TCTGCCAGGAATCTGTGACATC | Â | Â | Â |
PDGFRB | Â | 57 | 30 | 605 |
F | CTCAGGCCACGATGAAAGTG | Â | Â | Â |
R | GTCTCTGTGGACGCAGTTC | Â | Â | Â |
SLPI | Â | 57 | 22 | 239 |
F | AACCTGAGTGCCAGAGTGAC | Â | Â | Â |
R | AACGCAGGATTTCCCACACA | Â | Â | Â |
Maspin | Â | 57 | 23 | 378 |
F | AATGCCAAGGTCAAACTCTCC | Â | Â | Â |
R | GCATCCACAGAAAAGTCAGG | Â | Â | Â |
WFDC1 | Â | 59 | 26 | 314 |
F | GCTATGAGTGCCACATCCTGAG | Â | Â | Â |
R | ATCTCTGGGGTCTTGCTCTGCT | Â | Â | Â |
FBN2 | Â | 59 | 25 | 561 |
F | GAATGCTCCAATCCCAATGC | Â | Â | Â |
R | GCTGTCATCGTTCCCTTGAG | Â | Â | Â |
A2M | Â | 57 | 22 | 408 |
F | CATTGGCTATCTCAACACTGG | Â | Â | Â |
R | TGGTCCCCTTCTTGTGCTGTC | Â | Â | Â |
BCHE | Â | 59 | 25 | 520 |
F | CATTACACAGACTGGGTAGATG | Â | Â | Â |
R | CTTTCCACTCCCATTCTGCTTC | Â | Â | Â |
NEP | Â | 57 | 26 | 404 |
F | CTTCGATGACAATGGCAGAAAC | Â | Â | Â |
R | GCAAAGTCCCAATAATCCTGA | Â | Â | Â |
β-actin |  | 57 | 21 | 638 |
F | GGAGAAAATCTGGCACCACAC | Â | Â | Â |
R | CGTACAGGTCTTTGCGGATGT | Â | Â | Â |