Analysis of latexin expression in human immortalized and cancerous cells. (A) Specificity of 1G11, an anti-latexin monoclonal antibody, was tested by Western blot. The left panel is a coomasie stained gel and the right panel shows Western blot with GST and GST-latexin proteins. Expression of latexin (B) in GES-1 and MC cells and (C) in cytoplasmic (c) and nuclear (n) proteins extracted from the indicated cells was identified using Western blot with 1G11 antibody (1:1000 dilution). Actin was used as an internal control.