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Table 2 Details of antibodies used

From: The antiapoptotic gene survivin is highly expressed in human chondrosarcoma and promotes drug resistance in chondrosarcoma cells in vitro

Method Detected protein Primary antibody Concentration (μg/ml) Secondary antibody Concentration (μg/ml)
IHC Survivin pAB AF886 (R&D Systems) 5 Goat anti-rabbit immunoglobulin/biotinylated (DAKO) 4
IHC Survivin mAB clone 32.1 (Novus Biologicals) 6 Swine anti-mouse immunoglobulin/biotinylated (DAKO) 4
IHC Survivin pAB 500.201 (Novus Biologicals) 4 Goat anti-rabbit immunoglobulin/biotinylated (DAKO) 4
IHC Survivin mAB clone 60.11 (Novus Biologicals) 7 Swine anti-mouse immunoglobulin/biotinylated (DAKO) 4
IF Survivin pAB AF886 (R&D Systems) 2 Red fluorescent dye-labeled anti-rabbit immunoglobulin (Invitrogen) 1
IF Survivin mAB clone 60.11 (Novus Biologicals) 2 Red fluorescent dye-labeled anti-mouse immunoglobulin (Invitrogen) 1
IB Survivin pAB AF886 (R&D Systems) 1 Swine anti-rabbit immunoglobulins/HRP-conjugated (DAKO) 0,2
IB Survivin mAB clone 60.11 (Novus Biologicals) 2 Swine anti-mouse immunoglobulin/HRP-conjugated (DAKO) 0,2
  1. Primary and secondary antibodies used for immunohistochemistry, immunofluorescence, and immunoblotting are listed, with inclusion of sources, purpose, and antibody concentrations. IB - immunoblot, IHC - immunohistochemistry, IF - immunofluorescence;