Table 2 Details of antibodies used
Method | Detected protein | Primary antibody | Concentration (μg/ml) | Secondary antibody | Concentration (μg/ml) |
|---|---|---|---|---|---|
IHC | Survivin | pAB AF886 (R&D Systems) | 5 | Goat anti-rabbit immunoglobulin/biotinylated (DAKO) | 4 |
IHC | Survivin | mAB clone 32.1 (Novus Biologicals) | 6 | Swine anti-mouse immunoglobulin/biotinylated (DAKO) | 4 |
IHC | Survivin | pAB 500.201 (Novus Biologicals) | 4 | Goat anti-rabbit immunoglobulin/biotinylated (DAKO) | 4 |
IHC | Survivin | mAB clone 60.11 (Novus Biologicals) | 7 | Swine anti-mouse immunoglobulin/biotinylated (DAKO) | 4 |
IF | Survivin | pAB AF886 (R&D Systems) | 2 | Red fluorescent dye-labeled anti-rabbit immunoglobulin (Invitrogen) | 1 |
IF | Survivin | mAB clone 60.11 (Novus Biologicals) | 2 | Red fluorescent dye-labeled anti-mouse immunoglobulin (Invitrogen) | 1 |
IB | Survivin | pAB AF886 (R&D Systems) | 1 | Swine anti-rabbit immunoglobulins/HRP-conjugated (DAKO) | 0,2 |
IB | Survivin | mAB clone 60.11 (Novus Biologicals) | 2 | Swine anti-mouse immunoglobulin/HRP-conjugated (DAKO) | 0,2 |