Figure 4

Influence of survivin knockdown on proliferation and cell viability distribution of chondrosarcoma cells. The influences of RNA interference with survivin gene expression on cell viability and proliferation were measured by employing the MTT - assay (A) and by measuring BrdU incorporation (B). A: Cell viability was analyzed by MTT assay. Knockdown of survivin was performed at 0 hours and repeated at 48 hours in SW1353 (left) and Hs819.T (right). Significant reduction of viable cells compared to the untransfected control were seen in SW1353 after 48 hours and in Hs819.T at 72 hours.. Knockdown of GFP resulted in no significant alterations (Data not shown). The error bars represent +/-SEM. B: Proliferation of chondrosarcoma cell lines SW1353 and Hs819.T was measured by BrdU incorporation and subsequent detection employing a ELISA chemiluminescence immunoassay. Knock down was performed 24 hours prior to the incubation with BrdU. Knockdown of GFP resulted in no significant alterations. The error bars represent +/-SEM. A: Original results of one representative experiment are shown. B: Original results of three representative experiments are shown. P values less than 0.05 were considered significant (*).