Figure 6

Effect of huMETCAM/MUC18 expression on in vitro invasiveness of MCF7 cells (24 hours). The invasiveness of the pooled 2D and 3D clones of MCF7 was determined as 24 hours after seeding the cells described in "Materials and Methods" by coating with 150 μg of Matrigel and using a pore size of 8 μm. 7.5 to 15 μg/ml of the anti-huMCAM/MUC18 antibody (open columns) or the isotype control antibody (chicken IgY) (filled columns) was added to block the invasiveness of these clones. Means and standard deviations of six repeated values of the test are shown.