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Figure 5 | BMC Cancer

Figure 5

From: Up-regulation of METCAM/MUC18 promotes motility, invasion, and tumorigenesis of human breast cancer cells

Figure 5

Effect of huMETCAM/MUC18 expression on in vitro invasiveness of MCF7 cells (6 hours). The invasiveness of the pooled 2D and 3D clones of MCF7 was determined 6 hours after seeding the cells as described in "Materials and Methods". Since MCF7 clones exhibits a property of very low invasiveness, to observe more cells migrating to the bottom wells 6 hours after seeding in the invasiveness assay the bottom membrane of the top well was coated with 50 μg of Matrigel and a membrane with a pore size of 12 μm used [20]. 7.5 to 15 μg/ml of the anti-huMCAM/MUC18 antibody (open columns) or the isotype control antibody (chicken IgY) (filled columns) was added to block the invasiveness of these clones. Means and standard deviations of triplicate values of the test are shown.

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