Figure 13

Effect of huMETCAM/MUC18 expression on in vitro invasiveness of MDA-MB-231 and MDA-MB-468 cell lines (6 hours). The invasiveness of MDA-MB-231 and MDA-MB-468 cells was determined 6 hours after seeding the cells as described in "Materials and Methods". Similar to Figure 5, in order to observe more cells migrating to the bottom wells 6 hours after seeding in the invasiveness assay the bottom membrane of the top well was coated with 50 μg of Matrigel and a membrane with a pore size of 12 μm used [20]. 7.5 to 15 μg/ml of the anti-huMCAM/MUC18 antibody (open columns) or the isotype control antibody (chicken IgY) (filled columns) was added to block the invasiveness of these clones. Means and standard deviations of triplicate values of the test are shown.