Figure 1

HuMETCAM/MUC18 expression in four human breast cancer cell lines. 5 μg proteins of cell lysates were loaded in each lane in Western blot analysis to determine the level of METCAM/MUC18 in various cell lines. Cell lysates from a human melanoma cell line, SK-Mel-18, and a human prostate cancer cell line, DU 145, were used as positive controls (lanes 1-2), and that from a human prostate cancer cell line, LNCaP, as a negative control (lane 3). METCAM/MUC18 expression in cell lysates from four human breast cancer cell lines, MCF7, SK-BR-3, MDA-MB-231, and MDA-MB-468, were determined, as shown in lanes 4 to 7. The number under lane indicates the relative level of METCAMMUC18 of each cell line, assuming that in SK-Mel-28 as 100%. β-tubulin was used as the loading control.