ABI3 reduced tumor formation in nude mice and the ability to growth in semi-solid medium. (A) Representative animals that received either control cells (left panel) or cells expressing ABI3 (right panel) are shown. The arrows pointed out the formed tumors. (B) Tumorigenicity experiments are summarized in the graph showing mean ± SD of the volume of tumors after inoculation of ARO cells. (C) The percentage of colony formation in soft agar is significantly decreased in ARO cells expressing ABI3 in comparison with control cells. Experiments were performed in triplicates. *p < 0.05.