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Figure 4 | BMC Cancer

Figure 4

From: P27Kip1, regulated by glycogen synthase kinase-3β, results in HMBA-induced differentiation of human gastric cancer cells

Figure 4

Inhibition of GSK-3β overrides HMBA-induced CDK2 inhibition. SGC7901 cells were pre-treated with (+) or without (-) 10 mM LiCl (A) or 10 μM SB-415286 (B) for 30 min followed by combination treatment with 10 mM HMBA for 24 h. Protein extracts were immunoprecipitated with anti-CDK2 or anti-CDK4 antibodies. Resultant immune complexes were analyzed for CDK2 activity using histone H1 as substrate (upper panel) or for CDK4 activity using Rb as substrate (lower panel). Phosphorylated histone H1 or Rb protein signals were quantitated densitometrically and expressed as fold-change with respect to untreated control groups.

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