Skip to main content
Figure 1 | BMC Cancer

Figure 1

From: P27Kip1, regulated by glycogen synthase kinase-3β, results in HMBA-induced differentiation of human gastric cancer cells

Figure 1

Inhibition of GSK-3β attenuates HMBA-induced cell cycle arrest and SGC7901 cell differentiation. A, SGC7901 cells were pre-treated with or without 10 mM LiCl for 30 min followed by combination treatment with 10 mM HMBA for 24 h, followed with quantifying of DNA content by flow cytometry. B, SGC7901 cells were treated with HMBA (10 mM) for 24 h or 48 h and were prepared for western blotting analysis. C&E, SGC7901 cells were pre-treated with or without 10 mM LiCl or 10 μMSB-415286 for one hour followed by combination treatment with 10 mMHMBA for 24 h. proton pump expression status was determined via western blotting analysis. D&F, total RNA was extracted from cells and Q-RT-PCR analysis for proton pump mRNA expression was performed. (Data represent mean ± SD; * = p < 0.05 vs. control; * = p < 0.05 vs. HMBA alone.).

Back to article page