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Table 1 The type and distribution of mutations in BRAF and K-ras in the 186 adenocarcinoma and 16 adenoma tissues available from EPIC Norfolk.

From: Dietary, lifestyle and clinicopathological factors associated with BRAF and K-ras mutations arising in distinct subsets of colorectal cancers in the EPIC Norfolk study

  BRAFmutation
  Mutations in adenocarcinomas Mutations in adenomas Total
Codon 600: wildtype GTG    
GAG (Val to Glu) 29 0 29
Total 29 0 29
  K-ras mutation
  Mutations in adenocarcinomas Mutations in adenoma Total
Codon 12: wildtype GGT    
GCT (Gly to Ala) 7 1 8
GTT (Gly to Val) 6 1 7
GAT (Gly to Asp) 14 2 16
TGT (Gly to Cys) 4 1 5
AGT (Gly to Ser) 1 0 1
Undetermined* 1 0 1
Codon 13: wildtype GGC    
GAC (Gly to Asp) 6 1 7
TGC (Gly to Cys) 1 0 1
Codon 19: wildtype TTG    
GTT (Leu to Phe)** 1 0 1
Codon 20: wildtype ACG    
GCG (Thr to Ala)** 1 0 1
Codon 89: wildtype TCA    
TTA (Ser to Stop) 0 1 1
Total 42 7 49
  1. *: One adenocarcinoma sample was classified as mutated by both sequencing methods but the base change identified was not consistent. Dideoxysequencing identified the base change as G to A, pyrosequencing as G to C. Repeated sequencing using both methods did not resolve this and as such the base change was unclassified. **: The two mutations observed in codons 19 and 20 were in the same adenocarcinoma.