Table 1 Nucleotide sequences of primers used
From: Genetic and epigenetic silencing of the beclin 1gene in sporadic breast tumors
Name | Sequence (5'→3') | Purpose |
|---|---|---|
Bl-F | TGCAACCTTCCACATCT | RT-PCR |
Bl-R | TTCCACGGGAACACTG | RT-PCR |
β-actin-F | TCACCCACACTGTGCCCATCTACGA | RT-PCR |
β-actin-R | CAGCGGAACCGCTCATTGCCAATGG | RT-PCR |
Bl-QF | TCTGCCTTCCTCTGTAG | Q-PCR |
Bl-QR | TTCCACGGGAACACTG | Q-PCR |
18S-QF | ACATCCAAGGAAGGCAGCAG | Q-PCR |
18S-QR | TTCGTCACTACCTCCCCGG | Q-PCR |
Bl-Taqman | FAM-CACAGTGGACAGTTTGGCACAATCA-TAMRA | Q-PCR |
β-actin-Taqman | FAM-CAGCCGTGGCCATCTCTTGCTCGAA-TAMRA | Q-PCR |
18S-Taqman | FAM-CGCGCAAATTACCCACTCCCGA-TAMRA | Q-PCR |
Bl-bs1F | GTTTTTTAAAGTGTTGGAATTATAAG | Bisulfite first PCR |
Bl-bs1R | AACTCCTAATCCACAAACTCACAA | Bisulfite first PCR |
Bl-bs1F' | TTGTTGTTGTTTTGAGATGGAGTT | Bisulfite nested PCR |
Bl-bs1R' | AAAAATATAAAAACCAAAACC | Bisulfite nested PCR |
Bl-bs2F | GGGTTTGTGAGTTTGTGGATTAG | Bisulfite first PCR |
Bl-bs2R | AAAAAAAACTCCAATAAAAACC | Bisulfite first PCR |
Bl-bs2F' | AGTTTGTGGATTAGGAGTTTTTGTT | Bisulfite nested PCR |
Bl-bs2R' | TAAAAATTCCCAAACTCCCTTCTA | Bisulfite nested PCR |
Bl-bs3F | ATTTTAGAAGGGAGTTTGGGAATT | Bisulfite first PCR |
Bl-bs3R | TTAAACCCTTCCATCCCTAAAAC | Bisulfite first PCR |
Bl-bs3F' | TTTTGGGTTTTAAATTGTTTTTGTT | Bisulfite semi-nested PCR |
Bl-bs3R' | TTAAACCCTTCCATCCCTAAAAC | Bisulfite semi-nested PCR |
Bl-bs4F | ATATTGTGGATTTTTGAGAGTTTTT | Bisulfite first PCR |
Bl-bs4R | AAATCTTTCTTTTACTACTAAAAACTCTCT | Bisulfite first PCR |
Bl-bs4F' | TTGTAATTTTAGTATTTTGGGAGAT | Bisulfite nested PCR |
Bl-bs4R | CTCTATTACCCAAACTAAAATACAATAATA | Bisulfite nested PCR |
D17S579F | AGTCCTGTAGACAAAACCTG | Microsatellite analysis |
D17S579R | CAGTTTCATACCAAGTTCCT | Microsatellite analysis |