Figure 5

Expression of Trx in normal, BH, DCIS and IBC tissue. A. Tissues were subjected to IHC using a Trx-specific antibody. Representative slides are shown at 40× magnification. B. The relative mean intensity was determined from 6-8 images for each BH (n = 16), DCIS (n = 15) or IBC (n = 22) section and is shown graphically. ANOVA was used to detect statistical differences in the relative expression of BH, DCIS (p = 0.0039) and IBC (p < 0.0001) compared to normal mammary tissue, where normal mammary tissue is equal to one (dashed line). C. Cellular localization of Trx expression was determined for each tissue section, classified by disease state and shown graphically (N, nuclear; C, cytoplasmic; N+C, nuclear and cytoplasmic).