Figure 9
Immunofluorescent staining of ErbB1-4 receptors, phospho-ErbB2 and phospho-ErbB4 receptors in +SA cells after treatment with, γ-tocotrienol (γT 3 ), erlotinib (E), gefitinib (G), alone or in combination. +SA mammary tumor cells were plated at a density of 2 × 105 cells per well in 6 well plate (3 replicates/group) and incubated with control or treatment media for 4 days. The cells were then fixed with methanol and incubated with primary antibody directed against specific ErbB receptors followed by rhodamine conjugated secondary antibody, as described in the Methods section. Magnification is 200×.