Figure 5
Effects of combined γ-tocotrienol (γT 3 ), erlotinib (E), gefitinib (G), or trastuzumab (T) treatment on positive annexin V staining in +SA cells after a 4-day incubation period. +SA mammary tumor cells were plated at a density of 1 × 106 cells/100-mm culture dish and incubated with control or treatment media over a 4-day period. Afterwards, cells were isolated with trypsin, and 1 × 106 cells/group in triplicate were stained with annexin V, and then analyzed by flow cytometry. Viable cells display very low annexin V staining and appear in the marker 1 (M1) region, whereas cells undergoing apoptosis show very high annexin V staining and appear in the marker 2 (M2) region of the histogram.