(a) Real Time PCR: Significantly increasing gene expression of CD4, Foxp3, CD8, and CD25 in metastatic liver tissue on day 5, 10, 15 and 20 after intraportal injection of 1 × 10
5 CT26.WT cells (each group n = 7). Analysis was measured to control tissue (liver). (b) Immunohistochemistry: Increasing number (in %) of CD4, Foxp3, CD8, and CD25 at metastatic sites in the liver over time (day 5, 10, 15 and 20) after intraportal injection of 1 × 105 CT26.WT cells (each group n = 7). Lines with asterisks show significant increase during metastatic growth.