Detection of ZIC-like immunoreactivities in atypical and malignant meningioma. (A--C) Atypical meningioma with partially "blastic changes". (D--I) Anaplastic meningioma with geographic necrotic patterns. Each set of sections (A, B), (D, E), (F, G), and (H, I) is comprised of neighboring sections. (C) is the higher magnification of the boxed region in (B). (A) Hematoxylin and eosin staining. (B, C, D, F, H, J) Immunostaining with CXY2 antibody. (E, G) Immunostaining with anti-vimentin (VIM) antibody. (I, K) Immunostaining with anti-EMA antibody. Thick scale bar, 1 mm; thin scale bar, 100 μm.