Figure 2

Aberrations of chromosome 7 in MDA-MB-468CD44+/CD24 -/LOW. I) High resolution analysis by GeneChip Human Mapping 500 K arrays revealed a lot of aberrations occurring in MDA-MB-468 CD44+/CD24 -/LOW low as well high EGFR expressing cells. Based on DNAcopy and GLAD statistical package analysis statistical significance for a difference in gene copy could only be estimated for the indicated region containing the egfr gene on chromosome 7p. 2 II). Egfr FISH (green fluorescence) displays the egfr gene amplification based upon one chromosome with multiple egfr gene copies (white arrow; a) in MDA-MB-468 CD44+/CD24 -/LOW metaphase spreads. The amplicon is equal-spaced in a ladder like structure and the concerning telomere 7ptel (red fluorescence) is lost. Isochromosomes 7p are frequent findings (white arrows; b).