Figure 2

Quantitative and qualitative assessment of EGFR status. A-D: Immunohistochemical stain for EGFR of primary lung adenocarcinoma (magnification: 200×). A) No membranous or cytoplasmic stain. B) Weak mostly incomplete membranous stain combined with moderate cytoplasmic stain. C) Moderate mostly incomplete membranous stain combined with weak cytoplasmic stain. D) Strong mostly complete membranous stain combined with strong cytoplasmic stain. E and F: E) Fluorescence in-situ hybridization for EGFR (locus 7p12, red) and CEP7 (locus 7p11.1-q11.1, green) of primary lung adenocarcinoma showing no amplification of the EGFR-gen. F) Corresponding immunohistochemical stain for EGFR showing strong mostly incomplete membranous staining combined with moderate cytoplasmic staining.