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Table 4 IHC analysis confirms HAX-1 overexpression in tumors

From: HAX-1 overexpression, splicing and cellular localization in tumors

Patient

HAX-1

ER

 

Normal

Tumor

Tumor

 

Cytoplasm

Nucleus

Cytoplasm

Nucleus

Nucleus

1

0

-

2

-

+

2

0/1

-

1

-

-

3

1

-

1

-

-

4

0

-

0

-

-

5

0

-

1

-

-

6

0

-

1

-

-

7

1

-

0

+

+

8

0

-

2

+

+

9

0

-

1

-

-

10

2

-

2

-

+

11

1

-

1/2

-

+

12

0

-

2

+

+

13

0

-

2

-

-

14

0

-

2

+

+

15

0

-

3

-

-

  1. Immunohistochemical analysis of the 15 matched pairs of normal/tumor samples shows in most cases significant overexpression of HAX-1 in tumors. Up-regulation was observed in cytoplasm as well as nuclei of the tumor cells. Staining intensity was estimated as follows: negative = 0; weak = 1; intermediate = 2; and strong = 3. Nuclear staining was categorized as negative or weak (0-1:"-") and intermediate-strong (2-3: "+"). Strong nuclear HAX-1 staining was associated with strong ER immunopositivity. Fisher's exact test indicates that nuclear staining of HAX-1 and ER is significantly related (P = 0.01).