Table 3 Antigens more frequently reactive with CRC patient sera
From: Immunoscreening of the extracellular proteome of colorectal cancer cells
AG | Protein name | Gene symbol | PMF Profound | Accession | Seq Cov % | peptides matched (un-) | pI Seq | Seq_MW kDa | pI gel | MW gel kDa | Immune reactions % (no) | Diff fisher p < 0.05 | Exo somal | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CRC | control | |||||||||||||
39 | Not identified by MALDI, *1 but nano HPLC-ESI | PSMA1, | 217m | IPI00871889.1 | 18.6 | 4 | 6.6 | 30.2 | 6.8 | 27.8 | ||||
KLK6 | 117m | IPI00023845.1 | 12.7 | 3 | 7.9 | 26.9 | 6.8 | 27.8 | 48 (10) | 17 (4) | 0,0509 | yes | ||
31 | Phosphoglycerate mutase 1 (brain) | PGAM1 | 2.4 | gi|56081766 | 73.2 | 16 (23) | 6.7 | 28.8 | 7.5 | 28.1 | 66 (14) | 33 (8) | 0,0256 | yes |
2.4 | ||||||||||||||
74a | Lectin, mannose-binding 2 | LMAN2 | 2.3 | gi|16878112 | 38.5 | 11 (6) | 6.3 | 40.2 | ||||||
UV excision repair protein | RAD23B *1 | 124m | IPI00642549.2 | 7.4 | 3 | 5.2 | 42.3 | 5.7 | 32.4 | 29 (6) | 0 | 0,0067 | no | |
RAD23 homolog B*1 | ||||||||||||||
41 | syntenin isoform 3 | SDCBP | 71.5 | gi|55749515 | 53.2 | 10 | 7.9 | 32.3 | 7.6 | 32.6 | 38 (8) | 21 (5) | No 0,3234 | yes |
41 | syntenin isoform 1 | SDCBP | 71.4 | gi|55749490 | 53 | 10 | 6.8 | 32.4 | 7.6 | 32.6 | 38 (8) | 21 (5) | No 0,3234 | yes |
41 | syntenin [homo sapiens] | SDCBP | 71.4 | gi|2795863 | 53 | 10 | 6.8 | 32.4 | 7.6 | 32.6 | 38 (8) | 21 (5) | No 0,3234 | yes |
23 | Agrin precursor | AGRN | 0.6 | IPI00374563.2 | 0.6 | 7 | 6 | 214.7 | 5.2 | 23.6 | 19 (4) | 4 (1) | No 0,169 | no |
23 | AGRN_HUMAN Agrin | AGRN | 2.2s | gi|61218463 | 0.6 | 1 | 6 | 214.7 | 5.2 | 23.6 | 19 (4) | 4 (1) | No 0,169 | no |
23 | AGRN protein [Homo sapiens] carboxyterm of agrin precursor | AGRN | 65* | gi|21706410 | 59.1 | 7 (37) | 5.5 | 19.5 | 5.2 | 23.6 | 19 (4) | 4 (1) | No 0,169 | no |
49 | serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), | SERPINB1 | 2.2 | gi|62898301 | 30.3 | 12 (22) | 5.9 | 42.7 | 6.1 | 46.2 | 24 (5) | 4 (1) | No 0,0831 | yes |
49 | aminoacylase 1 [Homo sapiens] | ACY1 | 2.4 | gi|12653545 | 34.8 | 11 (3) | 5.8 | 45.9 | 6.1 | 46.2 | 24 (5) | 4 (1) | No 0,0831 | yes |
62a | Lectin, mannose-binding 2 | LMAN2 | 2.3 | gi|16878112 | 32.6 | 9 (33) | 6.3 | 40.2 | ||||||
Glyoxalase domain-containing protein 4*1 | Glod4 *1 | 244m | IPI00007102.3 | 12.1 | 5 | 7.7 | 55 | 5.4 | 33.1 | 42 (9) | 8 (2) | 0,0132 | no | |
(C17orf25) | IPI00792035.1 | 26.4 | 5 | 10 | 25.8 | |||||||||
24/24a | similar to huntingtin interacting protein 2; | HIP2 | 2 | gi|4885417 | 32.5 | 7 (21) | 5.2 | 22.5 | 5.0 | 23.6 | 24 (5) | 8 (2) | No | yes |
24/24a | B Chain B, Ubiquitin-Conjugating Enzyme E2-25 kDa | HIP2 | 2.0 | gi|60594412 | 37.6 | 7 (9) | 5.2 | 22.5 | 5.1 | 24.2 | 24 (5) | 8 (2) | No | yes |
2.4 | ||||||||||||||
24/24a | Crystal Structure Of Pi Class Glutathione Transferase | GSTP1 | 1.8 | gi|11514458 | 66 | 12 (19) | 5.3 | 32.1 | 5.1 | 24.2 | 24 (5) | 8 (2) | No | yes |
2.4 | gi|39654104 | |||||||||||||
70 | TALDO1 protein | TALDO1 | 2.4 | gi|48257056 | 32.1 | 10 (15) | 6.4 | 37.4 | 6.6 | 39.2 | v | |||
2.4 | ||||||||||||||
72 | The Ran-Gppnhp-Ranbd1 Complex | RANBD1 | 1.9 | gi|5107684 | 41.7 | 9 (22) | 9 | 23.2 | 7.8 | 26.4 | 19 (4) | 4 (1) | No | yes |
1.9 | ||||||||||||||
25b | Glutathione Transferase Mut | GSTP1 | 2.4 | gi|39654104 | 66.1 | 12 (19) | 5.3 | 23.1 | 5.5 | 24.6 | 19 (4) | 0 | No | yes |
69b | ALDOC protein | ALDOC | 2.4 | gi|41351364 | 37.6 | 11 (12) | 6.5 | 42.5 | 7.2 | 44.0 | 14 (3) | 0 | No | yes |
81b | glyoxalase I | GLO1 | 2.4 | gi|16198506 | 70.7 | 16 (17) | 5.1 | 20.7 | 4.6 | 24.8 | 9,5 (2) | 0 | No | yes* |