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Table 2 Identified antigens with most frequent reactivities (PMF spectra and mass lists are given in additional files 2 and 3)

From: Immunoscreening of the extracellular proteome of colorectal cancer cells

AG

Protein name

Gene symbol

PMF Profound

Accession

Seq Cov %

peptides matched (un-)

Seq pI

Seq_MW kDa

pI gel

MW gel kDa

immune reactions % (no)

Exo- somal

           

CRC

control

 

12

Triosephosphate isomerase 1 [Homo sapiens]

TPI1

2.4

gi|17389815

78.7

16

7.4

26.6

7.5

25.7

100 (21)

100 (24)

yes

18

Transferrin

TF

2.4

gi|15021381

35.8

26

6.7

77

6.4-7.1

75.8

100 (21)

100 (24)

yes

14

Enolase

ENO1

2.3

gi|4503571

33.4

13

7.4

47.2

7-7.6

55.0

100 (21)

96 (23)

yes

14

PA2G4 protein

PA2G4

2.1

gi|33879698

55.2

12

7.8

41.7

7-7.6

55.0

100 (21)

96 (23)

yes

17

Aldolase A

ALDOA

2.4

gi|4930291

53.4

16

8.3

44

8.5

45.0

76 (16)

75 (18)

no

2

succinate-ubiquinone oxidoreductase Ip subunit precursor

SDHB

1.7Sequest

gi|180917

6.1

1

9.1

30

7.8

16.5

81 (17)

75 (18)

no

2

UDHUP2 cystatin SN precursor [validated]-human

CST1

1.9

gi|2144579

50.4

8

7

16.4

7.8

16.5

81 (17)

75 (18)

no

10

peroxiredoxin 1; thioredoxin-dependent peroxide reductase 2; proliferation-associated gene A; natural killer-enhancingfactor A [Homo sapiens]

PRDX1

2.3

gi|32455266

59.3

12

8.3

22.1

8.1

22.9

57 (12)

71 (17)

no

10

lipocalin 2 (oncogene 24p3) [Homo sapiens]

LCN2

2.4

gi|49457137

65.2

14

9.6

22.5

8.1

22.9

57 (12)

71 (17)

no

3

A Chain A, Human Cyclophilin A Complexed With 2-Thr Cyclosporin

CYPA

2.3

gi|1431788

55.2

15

8.7

17.9

8.4

17.8

57 (12)

71 (17)

no

65a

stress-induced-phosphoprotein 1 (Hsp70/Hsp90)

STIP1

2.3

gi|5469884

20.8

11

6.4

62.6

6.3-7

66.0

57 (12)

71 (17)

yes

13

phosphoglycerate kinase 1/migration-inducing gene 10

PGK1

2.4

gi|41350401

61.4

25

8.3

44.7

8.2

45.0

62 (13)

67 (16)

yes

13

phosphoglycerate kinase 1 [homo sapiens]

PGK1

2.4

gi|129902

63.4

14

8.3

44.7

8.2

45.0

62 (13)

67 (16)

yes

19

Albumin (bovin)

ALB

2.4

gi|30794280

32.9

25

5.8

69.3

5.8-6

67.0

76 (16)

67 (16)

no

44a

enolase 1 variant

ENO1

2.3

gi|62897945

49.8

16

7.7

47.1

6.1

40.5

38 (8)

63 (15)

yes

44a

enolase 1 variant [Homo sapiens]

ENO1

2.3

gi|62896593

46.3

17

7.7

47.2

6.1

40.5

38 (8)

63 (15)

yes

30

Phosphoglycerate mutase 1 (brain) [Homo sapiens]

PGAM1

2.3

gi|56081766

72.8

15

6.7

28.8

7.1

28.3

62 (13)

54 (13)

yes

30

Phosphoglycerate mutase 1 (brain) [Homo sapiens]

PGAM1

2.2

gi|38566176

48.4

9

6.8

28.8

7.1

28.3

62 (13)

54 (13)

yes

11

Proteasome subunit MB1

PSMB5

2.3

gi|30582393

63

13

7.5

26.9

8.2

24.0

57 (12)

50 (12)

yes

26

peroxiredoxin 2 isoform b [Homo sapiens]

PRDX2

2.4

gi|33188452

54.4

9

6.2

16

5.6

22.9

43 (9)

46 (11)

yes

26

J Chain J, Thioredoxin Peroxidase B From Red Blood Cells

PRDX1

2.1

gi|9955016

57.4

14

5.6

21.6

5.6

22.9

43 (9)

46 (11)

yes

4

Cofilin 1 (non-muscle) [Homo sapiens]

CFL1

2.3

gi|15126676

46.4

7

8.3

18.5

8.7

15.9

(52 (11)

46 (11)

yes*

  1. * Not detected in overlay exosome sample and secretome master (see Figure 4), but detected in other experiments and literature