Figure 4

Down-regulation of iASPP inhibits proliferation of lung cancer cells through p53. Down-regulation of iASPP did not affect the proliferation of H1229 cells. (B) A549 cells and 95D cells had high p53 mRNA level, whereas H1299 cells had very low p53 mRNA levels. (C-D) The mRNA level of p21 and PUMA, which was downstream of the p53 pathway, was up-regulated when treated with iASPP shRNA in A549 cells. CON: blank control; NC, nonsense shRNA; KD: iASPP shRNA. Results represent the mean ± S.D. of three independent experiments.