FPET Pool | Gene | Difference in Mean CT
|
|---|
1 |
ATP5E
| 0.17 |
1 |
BGN
| 0.21 |
1 |
FAP
| 0.25 |
1 |
GADD45B
| 0.14 |
1 |
GPX1
| 0.16 |
1 |
INHBA
| 0.18 |
1 |
MK167
| 0.21 |
1 |
MYBL2
| 0.23 |
1 |
PGK1
| 0.28 |
1 |
UBB
| 0.22 |
1 |
VDAC2
| 0.19 |
1 |
MYC
| 0.26 |
2 |
ATP5E
| 0.08 |
2 |
BGN
| 0.08 |
2 |
FAP
| 0.11 |
2 |
GADD45B
| 0.13 |
2 |
GPX1
| 0.09 |
2 |
INHBA
| 0.05 |
2 |
MK167
| 0.1 |
2 |
MYBL2
| 0.15 |
2 |
PGK1
| 0.09 |
2 |
UBB
| 0.11 |
2 |
VDAC2
| 0.11 |
2 |
MYC
| 0.02 |
- FPE RNA pool 1 represents a sample with a RS in the low risk group (RS < 30) and FPE RNA pool 2 represents a sample with a RS in the high risk group (RS≥40). To determine reproducibility of the assay, (least square) mean CT and mean scores between different combinations of RT and qPCR Tecan workstations were contrasted. Shown is the largest pair-wise differences in (least square) mean non-normalized CT between Tecan workstations by gene for the two FPE RNA pools. Overall, the differences in mean CT between Tecan workstations across all 12 genes and the two FPE RNA pools were small, all ≤0.28 CT.
