FPET Pool | Score | Mean Score | Total SD |
|---|
1 | RS | 23.6 | 1.18 |
2 | RS | 43.6 | 1.38 |
1 | SGS | 8.2 | 0.03 |
2 | SGS | 8.5 | 0.04 |
1 | CCGS | 7.8 | 0.04 |
2 | CCGS | 6.9 | 0.05 |
1 | GADD45B | 5.5 | 0.13 |
2 | GADD45B | 6.6 | 0.11 |
- Two FPE RNA pools were created to provide homogeneous templates for measuring precision. FPE RNA pool 1 represents a sample with a RS in the low risk group (RS < 30) and FPE RNA pool 2 represents a sample with a RS in the high risk group (RS≥40). Using the standard 2 ng/well RNA equivalent, precision was assessed by estimating between-day, between-lot, between-7900 instrument, between-plate (within day), and within-plate variability components and total variability. Listed are the estimates of total variance for CCGS (normalized, aggregate CT values), SGS (normalized, aggregate CT values), GADD45B (normalized, aggregate CT values) and RS for each of the two FPET pools.
