Figure 4
LCC upregulates intracellular cAMP in C6 cells and this is required for CHX-induced C6 cell differentiation. A) Treatment of C6 cells with LCC elevated intracellular cAMP and cotreatment with Adenosine (Ado) blocked CHX effect. Experiments were carried out as described in Methods. Data are presented as means ± SEM (n = 3). * p < 0.01 (0 vs. 10 μg/ml CHX); ** p < 0.001 (10 μg/ml CHX with Ado vs. 10 μg/ml CHX without Ado). B) Adenosine blocked CHX-induced C6 morphological transformation. C6 cells (1.0×105 per well) were preincubated with or without Ado (100 μM) for 1 h and then cotreated with or without CHX (1 μg/ml) for 24 h as indicated. Phase contrast photos were taken as in Figure 1A. C) Adenosine blocked CHX-induced GFAP upregulation. Western immunostaining was carried out as in Figure 3C except cells were pretreated with or without Ado followed with cotreatment with or without CHX (1 μg/ml) and Ado for 24 h. D) Cell cycle arrest induced by CHX was not affected by Adenosine. Experiments were done as in Figure 1B except cells were pretreated with or without Ado. Data are presented as means ± SEM (n = 3).