Figure 3From: Induction of cell cycle arrest at G1 and S phases and cAMP-dependent differentiation in C6 glioma by low concentration of cycloheximide LCC induces C6 cell differentiation. A) LCC promotes morphological transformation of C6 cells that indicated cell differentiation. C6 cells were exposed to CHX at indicated concentration and to 8-cpt-cAMP (100 μM) for 24 h. Photos were taken as in Figure 1A (magnification, ×200). B-D) Treatment of C6 cells with LCC upregulates GFAP. In B), C6 cells cultured for 12 h in the absence (upper panels) or presence (lower panels) of CHX (1 μg/ml) were either photographed directly (left panels) or immunostained for GFAP (red) as described in Materials and Methods. Nuclei were stained with Hoechst 33258 as in Figure 1A. In C and D), GFAP expression was assessed in Western immunostaining analysis. Cells were treated with CHX at indicated concentration for 24 h (C) or 24 and 48 h (D). β-actin was used as loading control. E) Western blotting was done as in D) except anti-p-STAT3 was used. β-actin was used as loading control.Back to article page