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Figure 8 | BMC Cancer

Figure 8

From: Target enzyme mutations are the molecular basis for resistance towards pharmacological inhibition of nicotinamide phosphoribosyltransferase

Figure 8

Precipitation of NAMPT by sepharose linked TP201565. A: Chemical structure of linkable TP201565 analogue B: This figure shows western blots of NAMPT from 20 μg protein from HCT-116 lysate as well as NAMPT precipitated from HCT-116 lysate containing 20 μg total protein by TP201565 linked covalently to sepharose beads. The binding of NAMPT to beads is performed either with no inhibitor or in competition with a APO866 or b TP201565 at concentrations between 250 μM and 2.5 nM (in 10-fold steps).

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