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Figure 4 | BMC Cancer

Figure 4

From: Target enzyme mutations are the molecular basis for resistance towards pharmacological inhibition of nicotinamide phosphoribosyltransferase

Figure 4

Subcutaneous xenograft experiments of parental and resistant cell lines. A: Tumour doubling times of NAMPT resistant cell lines compared to tumour doubling times (TD) of parental cell lines (shown with SD). p-values are determined by t-tests. B: H&E stained sections of xenograft tumours at 200× magnification. a NYH and b NYH/CHS xenografts contained cells of various sizes and showed many mitoses. Tumours of c+e HCT-116, d HCT-116/APO866 and f HCT-116/TP201565 contain many fine connective tissue strands between tumour cells. Also, necrotic foci and densely growing epithelial cells are observed. Morphology of g PC-3 and h PC-3/TP201565 varied between areas of rounded cells and more spindle-shaped tumour cells. Many pyknotic (apoptotic) cell nuclei were also found. C: Cumulative survival of mice with HCT-116 and HCT-116/APO866 xenografts showing the time used by each mouse to grow its tumour to a size of 800 mm3. The mice were treated with 15-20 mg/kg APO866 or vehicle twice daily for 14 days (0-13, double arrow), where day 0 indicates the day tumours had a size of approx. 100 mm3. The result of log-rank analysis comparing APO866 treated mice with control mice in the respective xenografts (HCT-116: blue, HCT-116/APO866: red) is shown on the graph.

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