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Figure 5 | BMC Cancer

Figure 5

From: Elevated AKR1C3 expression promotes prostate cancer cell survival and prostate cell-mediated endothelial cell tube formation: implications for prostate cancer progressioan

Figure 5

Levels of VEGF mRNA expression in PC3-AKR1C3 transfectants. Total RNA was isolated from PC3-mock and PC3-AKR1C3 C1 transfectants as well as from PC3-AKR1C3 C1 clone treated with either 20 µM AG1024 or 10 µM LY294002. Target VEGF A, B, C, and D mRNA species were PCR amplified using isofrom-specific primer pairs. PCR products were separated by 1% agarose gel electrophoresis; and images of ethidium bromide-stained gels were acquired.

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