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Figure 2 | BMC Cancer

Figure 2

From: 1,1-Bis(3'-indolyl)-1-(p-substituted phenyl)methanes induce autophagic cell death in estrogen receptor negative breast cancer

Figure 2

Necrosis detection by PI staining and LDH release. MDA-MB-231 [A] and MCF-7 [B] cells were treated for 24 h with DMSO or 10 μM DIM-C-pPhCF3, DIM-C-pPhtBu or DIM-C-pPhC6H5. Two fluorescent DNA dyes Hoechst and propidium iodide, were loaded into cells and incubated on ice for 30 min and visualized using confocal microscopy as described in the Materials and Methods. The same microscopic field of MDA-MB-231 [A] or MDA-MB-453 [B] cells stained with Hoechst and propidium iodide are shown and are representative of other cell areas with the same treatment. At least 3 areas were scanned for each well and two wells were analyzed per treatment and per time point. Width of each field = 100 μm. LDH release after treatment with C-DIMs in MDA-MB-231 [C] and MDA-MB-453 cells [D]. Cells were treated for 48 h or 1 h with 0.1% Triton X and the supernatants were analyzed for LDH as described in the Materials and Methods. Triton X served as a positive for 100% cell lysis and LDH release. Results are presented as the means ± SE for at least three separate determinations for each treatment group. Statistical significance of treatments compared to DMSO (p < 0.05) are represented by an asterisk.

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