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Figure 2 | BMC Cancer

Figure 2

From: Activating transcription factor-3 (ATF3) functions as a tumor suppressor in colon cancer and is up-regulated upon heat-shock protein 90 (Hsp90) inhibition

Figure 2

Identification of signaling pathways for regulating ATF3. MAPK/Erk (UO), PI-3K/Akt (LY), SAPK (SP), or p38 (SB) inhibitors were used to identify signaling pathways involved in up-regulation of ATF3 in HCT116 colon cancer cells. A) Real-time PCR for ATF3 was performed after 1 hour incubation with indicated signaling inhibitors. Inhibition of SAPK (SP) significantly up-regulated ATF3 mRNA expression (P < 0.05). B) On a protein level, after 20 hours incubation with inhibitors, MAPK/Erk, SAPK and p38 pathways appeared to be involved in mediating ATF3 up-regulation. Bars: mean ± SEM

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BMC Cancer

ISSN: 1471-2407

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