TLR3, 4 and 9 gene expression measured by semiquantitative real time PCR in breast carcinomas. Upper panel (A), shows the percentage of TLRs expression in recurrence samples and without recurrence ones (WR). Lower panel,(B) shows the electrophoresis bands after real time-PCR performed on equal amounts of cDNA from each sample. The housekeeping used was β-actin. Data represent the mean SD of three independent experiments.