Figure 4From: KRAS analysis in colorectal carcinoma: Analytical aspects of Pyrosequencing and allele-specific PCR in clinical practice Evaluation of the sensitivity of the Pyrosequencing technology for detection of KRAS mutations. DNA from a KRAS codon 12 mutated tissue with a tumor cell content of 40% was serially diluted in wt DNA. Each dilution was analysed in triplicate by Pyrosequencing. The relative light units (RLU) for the mutation signal are presented.Back to article page