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Table 5 MALDI MS/MS characterization of proteins overexpressed in MGNT-derived cells forming spheres.

From: CD133, CD15/SSEA-1, CD34 or side populations do not resume tumor-initiating properties of long-term cultured cancer stem cells from human malignant glio-neuronal tumors

Entry SwissProt

Masse

Pi

% cover

Identification

RET1_HUMAN

15879.92

4,99

60

(P09455) Retinol-binding protein I

SORCN_HUMAN

21947.46

5,32

30

(P30626) Sorcin (22 kDa protein) (CP-22) (V19)

GSTP1_HUMAN

23438.07

5,44

27

(P09211) Glutathione S-transferase P (EC 2.5.1.18)

PRDX6_HUMAN

25002.19

6,02

25

(P30041) Peroxiredoxin 6 (EC 1.11.1.15)

ECHM_HUMAN

31823.30

8,34

29

(P30084) Enoyl-CoA hydratase

HSPB1_HUMAN

22825.51

5,98

66

(P04792) Heat-shock protein beta-1 (HspB1)

M6PBP_HUMAN

47189.00

5,30

53

(O60664) Mannose-6-phosphate receptor binding protein 1

Q96E67_HUMAN

40542.00

5,55

32

(Q96E67) ACTB protein (Fragment)

  1. The proteins listed in the table were only detected in 2D-SDS silver stained gels obtained from MGNT-derived cells forming spheres and not observed in the corresponding gel obtained with the same amount of proteins extracted from the original brain tumor. The proteins were identified by mass spectrometry analysis.