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Figure 5 | BMC Cancer

Figure 5

From: CD133, CD15/SSEA-1, CD34 or side populations do not resume tumor-initiating properties of long-term cultured cancer stem cells from human malignant glio-neuronal tumors

Figure 5

Intra-cerebral grafts. MGNT-derived cells forming spheres were grafted in the dorsal striatum of immunodeficient mice (inset in panel B). Mice were sacrificed from 12 to 22 weeks post-graft and brain sections immunostained. A. Hematoxylin-eosin staining was performed to identify tumor mass (inset: a cell undergoing mitosis within the tumor mass). B. Identification of human cells with an antibody specific of the human vimentin (brown). C-D. Double immunolabeling for vimentin (brown) and minichromosome maintenance 2 (mcm2, red nuclear labeling) identified cycling human cells (arrows). Cells were able to migrate at distance from the site of injection (D). Scale bar: 100 μm in B, 50 μm in A, 20 μm in C, D and inset in A. B-D: floxin counterstaining.

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