Immunocytochemical characterization of cells derived from MGNT. MGNT-derived cells form spheres in culture and express neural markers. Example of spheres from one tumor, TG1, cultured with FGF/EGF (A-D) or in the presence of 10% (E-F), or 0,5% FCS (G). A-D. In serum-free medium, cells within spheres expressed nestin (green, A), GFAP (red, B), Olig2 (red, C) and low levels of ß3-tubulin (green, D). E-G. After 14 days in serum-containing medium, the numbers of cells expressing Nestin decreased (green, E), The differentiated cells retained nuclear Olig2 expression (red, G). Some cells exhibited co-immunolabelling for GFAP (red) and ß3-tubulin (green, F). H. ß3-tubulin-immunoreactive cells in serum-free medium supplemented with B27. Nuclei were counterstained with DAPI or Topro. Scale bar = 40 μm. I. Quantification of cells expressing nestin, GFAP, Olig2 and ß3-tubulin in serum-free medium containing EGF and bFGF, in medium supplemented with 0.5 and 10% FCS, and in medium supplemented with B27.