p-ERK and SPRY levels in ccp1 over-expressing cell. (A) p-ERK was decreased in ccp1-expressing MEF in the steady state of culture under serum. (B) Cells were starved and treated with FGF2 for time indicated. Western Blotting was performed to examine levels of p-ERK. (C) A similar experiment was carried out for a treatment with serum. (D) MEF expressing ccp1 were cultured in absence of serum for 24 h and treated with 50 ng/ml FGF2 in the presence of 10 ug/ml heparin for time indicated. Levels of SPRY were analysed by Western Blotting. 7.5% SDS-PAGE gels were used. Densitometry analysis showed the fold changes for each blot, showing changes in p-ERK1/2 (A-C) and Spry2 (D).