Figure 6

Cell proliferation promoted by ccp1 was partially reduced by addition of MEK inhibitor. (A) MEF cells were treated with various concentrations of U0126 in the presence of serum for 24 h and BrdU assay was performed. The concentration of 20 μM was chosen. (B) Cells expressing ccp1 were serum starved overnight, treated with 20 μM of U0126 and BrdU assay was performed. (C) Cells expressing ccp1 were serum starved overnight, treated with 50 ng/ml FGF2 and 10 μg/ml heparin, 20 μM of U0126 or combinations as indicated for 24 h and BrdU assay was performed. (D) Cells expressing ccp1 were treated with 10% serum, 20 μM of U0126 or combinations as before. (E) Control MEF expressing eGFP and MEF expressing ccp1 were treated with 20 μM of U0126 for 24 h and Western blotting was performed to detect the level of p-ERK. Densitometry analysis showed the fold changes in p-ERK1/2.