Figure 5

Stable expression of the ccp1 protein reduced apoptosis in the absence of serum. TUNEL assay was performed in SK-N-SH cells stably expressing ccp1/eGFP protein and eGFP alone as a control. Cells were cultured in the absence of serum for three days. The number of cells that went through apoptosis increased in the control, up to 15.9 ± 2.6% of total number of cells visualized by DAPI on the third day. In contrast, apoptotic cells in cells expressing ccp1/eGFP protein remained low, up to 2.3 ± 1.8% (n = 3) (**p < 0.005).