Figure 4

Cell proliferation was inhibited when ccp1 expression was knocked down in NIH3T3 cells. (A) Ccp1 expression was knocked down in NIH3T3 cells using specific ccp1-siRNA (siRNA1 and siRNA2). Ccp1 expression after silencing was assessed by RT-PCR. Actin amplification was performed to show equal sample loading. Densitometry analysis showed the fold changes. (B) BrdU assay performed in absence of serum showed that cell proliferation in cells knocked down with ccp1 decreased compared to the scramble siRNA-treated cells (n = 3, ** = p < 0.005). Cells transfected with Scramble siRNA did not show significant reduction in cell proliferation.