(A), Immunohistochemical determination of p-Smad2. P-Smad2 was found in the nuclei of cancer cells. The Immunoreactive intensity score of p-Smad2 staining in tumour cells was evaluated using the positive and negative controls. Score 3 means high intensity as same as the positive control. Immunoreactivity, score 1 = weak, score 2 = moderate, and score 3 = intense. (×400). (B), The overall survival of patients based on p-Smad2 expression. The Kaplan-Meier survival curve shows the overall survival in relation to the p-Smad2 expression in 135 patients with gastric carcinoma. A statistically significant difference in the survival was observed between the high p-Smad2 groups and low p-Smad2 groups (p = 0.035; log-rank,).