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Figure 7 | BMC Cancer

Figure 7

From: Localization of uPAR and MMP-9 in lipid rafts is critical for migration, invasion and angiogenesis in human breast cancer cells

Figure 7

Time course studies of uPAR before and after lipid raft disruption in MDA-MB-231 cells. MDA-MB-231 cells were left untreated or pretreated with 7.5 mM MβCD for one hour at 37°C; washed; fixed with 1% paraformaldehyde at 1, 8 and 24 hrs; permeabilized; incubated with primary antibodies against uPAR and LAMP-1 overnight at 4°C; washed; incubated with appropriate Alexa Fluor secondary antibodies; and mounted with an anti-fading agent containing DAPI. The cells were imaged under an Olympus fluoview confocal microscope.

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