Figure 6
From: Potent inhibition of rhabdoid tumor cells by combination of flavopiridol and 4OH-tamoxifen
Effect of p53 knock-down on flavopiridol- and 4OH-Tam-induced cell cycle arrest and apoptosis: A. Immunoblot analysis to indicate siRNA mediated knock-down of p53 in MON cells treated with flavopiridol and 4OH-Tam for two days B. Percentage of diploid MON cells at various stages of cell cycle after treatment with control (Cy3) siRNA followed by treatment with flavopiridol and 4OH-Tam for two days. Note the lack of G 2 arrest upon drug treatment of p53-knock-down cells. C. Percentage of diploid MON cells at various stages of cell cycle after p53 knockdown (using siRNA to p53) and treatment with flavopiridol and 4OH-Tam for two days. D. Percentage of MON cells in sub-G1 after transfection with control (Cy3) or p53 siRNAs and exposed to flavopiridol and 4OH-Tam for two days.