Table 2 Sequences of forward and reverse primers (5'-3') used for PCR and sequencing
Gene | Exon | Forward primer | Reverse primer |
|---|---|---|---|
EGFR | 18 | TCAGAGCCTGTGTTTCTACCAA | TGGTCTCACAGGACCACTGATT |
EGFR | 19 | AAATAATCAGTGTGATTCGTGGAG | GAGGCCAGTGCTGTCTCTAAGG |
EGFR | 20 | ACTTCACAGCCCTGCCGTAAAC | ATGGGACAGGCACTGATTTGT |
EGFR | 21 | GCAGCGGGTTACATCTTCTTTC | CAGCTCTGGCTCACACTACCAG |
PIK3CA | 9 | GGGAAAAATATGACAAAGAAAGC | CTGAGATCAGCCAAATTCAGTT |
PIK3CA | 20 | CTCAATGATGCTTGGCTCTG | TGGAATCCAGAGTGAGCTTTC |
KRAS | 1 | GGTGGAGTATTTGATAGTGTATTAACC | AGAATGGTCCTGCACCAGTAA |
BRAF | 15 | TGCTTGCTCTGATAGGAAAATG | AGCATCTCAGGGCCAAAAAT |
PTEN | 5 | GCAACATTTCTAAAGTTACCTA | CTGTTTTCCAATAAATTCTCA |
PTEN | 6 | CATAGCAATTTAGTGAAATAACT | GATATGGTTAAGAAAACTGTTC |
PTEN | 7 | CAGTTAAAGGCATTTCCTGTG | GGATATTTCTCCCAATGAAAG |
PTEN | 8 | CTCAGATTGCCTTATAATAGTC | AACTTGTCAAGCAAGTTCTTC |
PTEN | 9 | GTTCATCTGCAAAATGGA | GGTAATCTGACACAATGTCCTA |
HER2 | 18 | GTGAAGTCCTCCCAGCCCGC | CTCCCATCAGAACTGCCGACC |
HER2 | 19 | TGGAGGACAAGTAATGATCTCCTGG | AGACCAGAGCCCAGACCTG |
HER2 | 20 | GCCATGGCTGTGGTTTGTGATGG | ATCCTAGCCCCTTGTGGACATAGG |
HER2 | 21 | GGACTCTTGCTGGGCATGTGG | CCACTCAGAGTTCTCCCATGG |
HER2 | 22 | CCATGGGAGAACTCTGAGTGG | TCCCTTCACATGCTGAGGTGG |
HER2 | 23 | AGACTCCTGAGCAGAACCTCTG | AGCCAGCACAGCTCAGCCAC |