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Table 4 Expression and promoter hypermethylation analysis of Cyclin D 2 and PTCH1 gene in astrocytic tumor samples

From: Regulation of sonic hedgehog-GLI1 downstream target genes PTCH1, Cyclin D2, Plakoglobin, PAX6 and NKX2.2 and their epigenetic status in medulloblastoma and astrocytoma

Astrocytic tumor samples Grade and Sample ID Expression of Cyclin D2 in tumor samples Methylation by MSP in Cyclin D2 promoter Methylation by MCA-Meth in Cyclin D2 promoter Methylation by both techniques in Cyclin D2 promoter No hypermethylation in Cyclin D2 promoter Expression of PTCH1 in tumor samples Methylation of PTCH1 promoter by MCA-Meth
GBM(3) -   -
AIII(4) ++     +  
GBM(5) -     +/-  
GBM(6) -     +/-  
AIII(7) +     +/-  
GBM(8) -     ++  
GBM(9) +     -  
AIII(10) -     +/-  
GBM(11) ++     +/-  
GBM(12) +     +  
GBM(13) -   +
AIII(14) ++     -  
GBM(15) -     +/-  
AIII(16) ++     +/-  
GBM(17) -     ++  
GBM(18) -   +  
AIII(19) +     +  
AII(20) +     +  
AIII(21) -     -
GBM(22) -     -  
GBM(23) -     +  
GBM(24) -   +  
AI(25) ND     ND  
GBM(26) ND     ND  
GBM(27) ND     ND  
GBM(28) ND     ND  
GBM(29) ND     ND  
GBM(30) +     +  
GBM(31) ND     ND  
GBM(32) ND     ND  
AI(34) ND     ND  
AIII(35) ND     ND  
GBM(36) ND     ND  
GBM(41) ND     ND  
GBM(43) +     -  
GBM(44) ND     ND  
GBM(45) ND     ND  
GBM(46) ND     ND  
GBM(47) ND     ND  
GBM(48) ND     ND  
GBM(49) ND     ND  
AII(50) ++     -  
GBM(51) -     -  
GBM(52) ++     +  
  1. -:no expression; +/-: low expression; +: expression; ++: high expression; √: yes; nd: not determined