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Table 4 Expression and promoter hypermethylation analysis of Cyclin D 2 and PTCH1 gene in astrocytic tumor samples

From: Regulation of sonic hedgehog-GLI1 downstream target genes PTCH1, Cyclin D2, Plakoglobin, PAX6 and NKX2.2 and their epigenetic status in medulloblastoma and astrocytoma

Astrocytic tumor samples Grade and Sample ID

Expression of Cyclin D2 in tumor samples

Methylation by MSP in Cyclin D2 promoter

Methylation by MCA-Meth in Cyclin D2 promoter

Methylation by both techniques in Cyclin D2 promoter

No hypermethylation in Cyclin D2 promoter

Expression of PTCH1 in tumor samples

Methylation of PTCH1 promoter by MCA-Meth

GBM(3)

-

√

√

√

 

-

√

AIII(4)

++

   

√

+

 

GBM(5)

-

   

√

+/-

 

GBM(6)

-

√

   

+/-

 

AIII(7)

+

   

√

+/-

 

GBM(8)

-

√

   

++

 

GBM(9)

+

   

√

-

 

AIII(10)

-

√

   

+/-

 

GBM(11)

++

   

√

+/-

 

GBM(12)

+

   

√

+

 

GBM(13)

-

√

√

√

 

+

√

AIII(14)

++

 

√

  

-

 

GBM(15)

-

√

   

+/-

 

AIII(16)

++

√

   

+/-

 

GBM(17)

-

 

√

  

++

 

GBM(18)

-

√

√

√

 

+

 

AIII(19)

+

 

√

  

+

 

AII(20)

+

 

√

  

+

 

AIII(21)

-

√

   

-

√

GBM(22)

-

 

√

  

-

 

GBM(23)

-

 

√

  

+

 

GBM(24)

-

√

√

√

 

+

 

AI(25)

ND

   

√

ND

 

GBM(26)

ND

   

√

ND

 

GBM(27)

ND

   

√

ND

 

GBM(28)

ND

   

√

ND

 

GBM(29)

ND

   

√

ND

 

GBM(30)

+

√

   

+

 

GBM(31)

ND

   

√

ND

 

GBM(32)

ND

   

√

ND

 

AI(34)

ND

   

√

ND

 

AIII(35)

ND

   

√

ND

 

GBM(36)

ND

   

√

ND

 

GBM(41)

ND

   

√

ND

 

GBM(43)

+

√

   

-

 

GBM(44)

ND

   

√

ND

 

GBM(45)

ND

   

√

ND

 

GBM(46)

ND

   

√

ND

 

GBM(47)

ND

   

√

ND

 

GBM(48)

ND

√

   

ND

 

GBM(49)

ND

√

   

ND

 

AII(50)

++

   

√

-

 

GBM(51)

-

√

   

-

 

GBM(52)

++

   

√

+

 
  1. -:no expression; +/-: low expression; +: expression; ++: high expression; √: yes; nd: not determined